Pegasus HRT GC and GCxGC-TOFMS

LECO Pegasus High-Resolution GC-TOF-MSClose up on LECO Pegasus GC HRT with LPAL autoloaderScientist adjusting GCxGC column in LECO Pegasus HRT

Redefining High-Resolution Analysis

Never miss another clue with the Pegasus HRT high-resolution GC-TOFMS (gas chromatography time-of-flight mass spectrometry) platform. The HRT delivers <1 ppm mass accuracy and resolving power upwards of 50,000 without sacrifices to sensitivity, acquisition speed, or spectral fidelity. Exceptional mass accuracy and soft ionization capabilities enable accurate formula determination for elucidating the identity of unknown analytes.

For the most complex mixtures, pair the HRT with LECO’s QuadJet thermal modulator for enhanced separation power and identification accuracy. The HRT empowers deeper insights for environmental studies, petroleum characterization, metabolic profiling, and much more.


Why Choose the Pegasus HRT Series?

Cutaway rendering of Pegasus HRT showing ion path

Ultra-High Resolution for Confident Identification

Folded Flight Path (FFP) time-of-flight (TOF) technology delivers resolving power over 50,000 to easily isolate analyte signals from matrix.

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Superb Mass Accuracy Improves Identifications

Routinely achieve sub-ppm mass accuracy for confident molecular formula calculations.

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Capture Everything in One Run

Acquire full-spectrum data at speeds up to 200 spectra per second, ideal for GCxGC coupling and complex, non-target analyses.

LECO Multi-Mode Source

Innovative, Flexible Ionization Source

The HRT comes standard with the Multi-Mode Source (MMS)—select Electron Impact (EI), Positive Chemical Ionization (CI), or Negative Chemical Ionization (NCI) with the click of a button.



Core Analyses


Featured Application Notes

water in motion

Identifying Disinfection Byproducts in Treated Water

This application note demonstrates the use of LECO’s Pegasus GC-HRT for non-target analysis of swimming pool and hot tub water. Known and unknown disinfection byproducts are identified using high-resolution deconvolution, accurate mass EI and CI data, and advanced ChromaTOF tools to support comprehensive chemical characterization of complex water matrices.
pattern representing chemical extracts

Identification of Components of Polymer Extracts by Gas Chromatography with EI and CI High Resolution Time-of-Flight Mass Spectrometry

This note details a workflow for characterizing complex polymer extracts using high-resolution GC-TOFMS. By leveraging both Electron Impact (EI) and Chemical Ionization (CI), it overcomes the limitations of standard library matching to identify degradants and adulterants.
Electronics e-waste

Discovery of Environmental Pollutants at an Electronic Waste Recycling Facility by Pegasus GC-HRT 4D

This study utilizes comprehensive two-dimensional gas chromatography coupled with high-resolution time-of-flight mass spectrometry (GCxGC-HRT) to discover emerging organic pollutants in e-waste facilities. It highlights the power of 4D thermal modulation and sub-ppm mass accuracy in characterizing complex environmental extracts.

How it works

The LECO Pegasus HRT is a gas chromatography–mass spectrometry (GC‑MS) system designed for the analysis of complex organic mixtures. It combines gas chromatography for chemical separation with high‑resolution time‑of‑flight mass spectrometry (HR‑TOFMS) for compound detection and identification. The system is commonly paired with comprehensive two‑dimensional gas chromatography (GCxGC) to increase separation capacity for samples containing many co‑eluting components.

After chromatographic separation, analytes enter the mass spectrometer ion source, where they are typically ionized by electron ionization (EI). Soft ionization techniques like Chemical Ionization (CI) and Negative Chemical Ionization (NCI) can also be used to preserve molecular ions for improved elemental composition determination. With LECO’s Multi-Mode Source (MMS) for the HRT, analysts can switch between the three ionization modes with a simple software toggle.

The resulting ions are accelerated into a time‑of‑flight analyzer, where they are separated based on their mass‑to‑charge ratio by measuring the time required to travel a fixed distance. LECO’s unique Encoded Frequency Pushing (EFP) maximizes TOF duty cycle, instrument sensitivity, and spectral quality.

The Pegasus HRT employs the Folded Flight Path (FFP), which transmits ions for either 20 or 40 meters before reaching the detector, depending on the acquisition mode selected. Use of an ultra-long flight path ensures reliable detection of all ions that is not sensitive to atmospheric conditions or sample concentration.

Ions are detected by a fast detector, and the resulting signals are deconvoluted into accurate‑mass spectra across the full mass range for every chromatographic peak. Because the system collects full spectral data without mass filtering, both known and unknown compounds can be evaluated post‑analysis without re‑running samples.


Multi-Mode Source

The LECO Multi-Mode Source (MMS) is a versatile ionization platform designed to streamline complex characterization by integrating multiple ionization techniques into a single, high-performance source. It allows users to switch seamlessly between Electron Ionization (EI), Positive Chemical Ionization (PCI), and Electron Capture Negative Ionization (ECNI) via simple software commands. This eliminates the need for manual hardware changes, ensuring perfect retention time alignment across different modes and providing the high mass accuracy required for both molecular and fragment ions in a single workflow.

LECO Multi-Mode Source
Cutaway rendering of Pegasus HRT showing ion path

Folded Flight Path

LECO’s patented Folded Flight Path (FFP) maximizes resolving power and mass accuracy while maintaining a compact instrument footprint. By extending the effective ion flight length through a folded geometry, the system achieves high resolution and precise mass measurements without sacrificing stability or speed. This design minimizes spatial requirements while enhancing separation of closely spaced mass peaks, supporting confident identification of compounds even in highly complex or trace‑level analyses.


Instrument Details

Specifications

Ionization Source
Electron Ionization or Multi-Mode Source (single source for EI/CI/NCI modes)
Mass Range
10–1,500 m/z
Mass Resolution (High Resolution Mode)
> 25,000 at m/z 219
Mass Resolution (Ultra-High Resolution Mode)
> 50,000 at m/z 219
Mass Accuracy
< 1 ppm independent of acquisition rate
Spectral Acquisition Rate
1–200 spectra/second
Instrument Detection Limit (IDL)
< 85 fg for 8 replicates of 100 fg on column (EI mode)
Precision
< 5% RDS for 15 injections of 100 pg OFN
Linear Dynamic Range
4+ orders of magnitude

Models

Pegasus HRT
Pegasus HRT 4D

Model Comparison

Feature / Capability
HRT
HRT4D (QuadJet Thermal Modulator)
GC Configuration
1-dimensional chromatograph
Comprehensive multidimensional chromatograph, dual-stage thermal modulator with secondary column oven, available in LN2 cryogenics or cryogen-free versions
Key Features
Flexible GC-MS configuration with high resolution, accurate mass time-of-flight (TOF) mass analyzer and Multi-Mode Source (EI/CI/NCI)
Market-leading GCxGC performance, combined with HRAM MS and Multi-Mode Source (EI/CI/NCI)
Chromatographic Resolution
Standard
Excellent (avg. FWHM 50–100 ms)
Volatility Range
Only limited by column set
C4 (butane) through column set limit (LN2) / C8 (octane) through column set limit (consumable free)

Options

Pyrolysis and Thermal Desportion Solutions


Instrument Support

Ensure reliable instrument performance with extensive support, training, and maintenance services. Need tailored support for your mass spectrometers? Our experts are standing by.




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